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pdonr223 kraswt  (Addgene inc)


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    Structured Review

    Addgene inc pdonr223 kraswt
    Pdonr223 Kraswt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pdonr223+kraswt/pDONR223_KRAS_WT+(Plasmid+%2381751)/pm39255801-274-5-7
    Average 93 stars, based on 5 article reviews
    pdonr223 kraswt - by Bioz Stars, 2026-09
    93/100 stars

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    other:

    Article Title: Targeting Ras-, Rho-, and Rab-family GTPases via a conserved cryptic pocket.
    Article Snippet: KRAS or NRASmutagenesis on the aforementioned inducible vectors was performed with the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent).

    Construct:

    Article Title: Targeting Ras-, Rho-, and Rab-family GTPases via a conserved cryptic pocket.
    Article Snippet: .. The plasmids pCW57.1 (Addgene 41393), pDONR223 KRASWT (Addgene 81751), and pDONR223 NRASWT (Addgene 82151) were used to generate doxycycline-inducible KRAS and NRAS constructs as previously described.23 Briefly, mCherry was Gibson cloned from pHR-SFFV-KRAS-dCas9-P2A-mCherry (Addgene 60954) construct to the N-terminus of KRAS or NRAS on the pCW57.1 backbone. .. KRAS or NRASmutagenesis on the aforementioned inducible vectors was performed with the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent).

    Clone Assay:

    Article Title: Targeting Ras-, Rho-, and Rab-family GTPases via a conserved cryptic pocket.
    Article Snippet: .. The plasmids pCW57.1 (Addgene 41393), pDONR223 KRASWT (Addgene 81751), and pDONR223 NRASWT (Addgene 82151) were used to generate doxycycline-inducible KRAS and NRAS constructs as previously described.23 Briefly, mCherry was Gibson cloned from pHR-SFFV-KRAS-dCas9-P2A-mCherry (Addgene 60954) construct to the N-terminus of KRAS or NRAS on the pCW57.1 backbone. .. KRAS or NRASmutagenesis on the aforementioned inducible vectors was performed with the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent).



    Similar Products

    93
    Addgene inc pdonr223 kraswt
    Pdonr223 Kraswt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pdonr223+kraswt/pDONR223_KRAS_WT+(Plasmid+%2381751)/pm39255801-274-5-7
    Average 93 stars, based on 1 article reviews
    pdonr223 kraswt - by Bioz Stars, 2026-09
    93/100 stars
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